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GENETIC CHARACTERIZATION AND CERTIFICATION OF B. SUBTILIS STRAINS – ANTAGONISTS OF PHYTOPATHOGENIC BACTERIA AND FUNGI
Terletskiy Valery Pavlovich1, Tyshchenko Valentina Ivanovna2, Novikova Irina Igorevna3, Bojkova Irina Vasiljevna4
1Russian Research Institute of Farm Animal Genetics and Breeding, D.Sc. in biology, senior researcher of the Laboratory of Molecular Cytogenetics
2Russian Research Institute of Farm Animal Genetics and Breeding, Cand.Sc. in biology, senior researcher of the Laboratory of Molecular Cytogenetics
3All Russian Research Institute of Plant Protection, D.Sc. in biology, senior researcher of the Laboratory of Microbiology
4All Russian Research Institute of Plant Protection, Cand.Sc. in biology, senior researcher of the Laboratory of Microbiology
1Russian Research Institute of Farm Animal Genetics and Breeding, D.Sc. in biology, senior researcher of the Laboratory of Molecular Cytogenetics
2Russian Research Institute of Farm Animal Genetics and Breeding, Cand.Sc. in biology, senior researcher of the Laboratory of Molecular Cytogenetics
3All Russian Research Institute of Plant Protection, D.Sc. in biology, senior researcher of the Laboratory of Microbiology
4All Russian Research Institute of Plant Protection, Cand.Sc. in biology, senior researcher of the Laboratory of Microbiology
Abstract
The microorganism genotyping technique developed earlier which is based on the double digest and selective label approach has been developed for identification of B. subtilis strains. The procedure of genomic DNA extraction from this microorganism has been optimized. The method can be recommended for genetic certification and confirmation of origin of commercially used bacterial strains.
Keywords: B. subtilis, DNA digestion, genotyping, labeling, phytopathogens, restriction enzymes
Category: 03.00.00 Biology
Article reference:
Genetic characterization and certification of B. subtilis strains - antagonists of phytopathogenic bacteria and fungi // Modern scientific researches and innovations. 2015. № 6. P. 1 [Electronic journal]. URL: https://web.snauka.ru/en/issues/2015/06/54397
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